Table 2.

Primers used for methylation-specific PCR

Gene	Primer	Sequence (5′ –> 3′)	Position	Length (bp)	CpG sites
HOXD1	Forward	CCCCGTTGTAGGTAAATTCGTC	Chr2:177054528–177054672	144	10
	Reverse	GGGACTATCTCGATACGCCGA
HOXC10	Forward	TATTTGACGCGAGAGCGTCG	Chr12: 54383074–54383217	147	7
	Reverse	TTAAAATTAAAAATCAATTCCCG