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Coomassie brilliant blue-stained SDS-polyacrylamide gel showing material representative of E. coli membrane fractions used to immunize hamsters. Lanes: 1 and 2, E. coli XL1-Blue with plasmids pMMB66 and pMMB66-OmpL1, respectively; 3 and 4, E. coli JM109(DE3) with plasmids pET15b and pET15b-LipL41*, respectively. Locations of the OmpL1 doublet in lane 2 and of LipL41 in lane 4 are shown (arrowheads). Positions of molecular size markers (M) are given in kilodaltons.
