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. 2022 Nov 29;15:38. doi: 10.1186/s13072-022-00472-5

Fig. 2.

Fig. 2

Depiction of transgene constructs. pBYU1(the control construct) with the unc-54 minimal enhancer (blue). pBYU9, pBYU14, and pBYU18 have putative nucleosome positioning [601(orange) or Trifonov (purple)] elements around the unc-54 minimal enhancer. pBYU28, pBYU29, pBYU41, and pBYU44 were used to test putative repelling elements; pBYU28 with 20 bp poly-A repeats (red), pBYU29 with the 70 bp PRS-322 sequence (black), pBYU41 with forward- and reverse-oriented 5 bp poly-T sequences (turquoise), and pBYU44 with an AAAAAcAAAAA sequence (dark blue) downstream of the unc-54 minimal enhancer (blue)