Table 1.
Transgene construct purposes and modifications
| Purpose | Modification/insert | Insert location/direction | Plasmid | Worm strain | |
|---|---|---|---|---|---|
| Upstreama | Downstreamb | ||||
| Control | Minimal unc54 enhancer | – | – | pBYU1 | BYU1 |
| Positioning | 601 | Forward | – | pBYU14 | BYU10 |
| 601 | Forward | Forward | pBYU18 | BYU12 | |
| Trifonov | Forward | Forward | pBYU9 | BYU6 | |
| Repelling | A(5)-C-A(5) | – | Reverse | pBYU44 | BYU19 |
| T(5) | Forward | Reverse | pBYU41 | BYU15 | |
| A(20) | Forward | Forward | pBYU28 | BYU14 | |
| PRS-322 | Forward | Reverse | pBYU29 | BYU13 | |
aInserted in the HindII site upstream of the unc-54 minimal enhancer
bInserted in the NheI site downstream of the unc-54 minimal enhancer