Table 4.
Sequences of primers used in plasmid construction and biotinylated DNA probe generation
| Primer name | Primer sequence |
|---|---|
| SJ-BYU-1 | AAGCTTATCCCATTCTCTCATCAATTGAGTGGGATGAGGCTATCTCTGCCTCTCTTCTGA |
| SJ-BYU-2 | GCTAGCGTCATCCACAGTGTAATGTAAGATGGTTCAGAGATTCAGAAGAGAGGCAGAGAT |
| CK-SJ-5 | ACTAGCAAGCTTGGAGATCCCTCGAAAATTTC |
| CK-SJ-6 | GTGCTCAAGCTTGGAAATCTCCGGGGAATTTC |
| CK-SJ-7 | ACTAGCGCTAGCGGAGATCCCTCGAAAATTTC |
| CK-SJ-8 | GTGCTCGCTAGCGGAAATCTCCGGGGAATTTC |
| HindIII601 F | AAGCTTACAGGATGTATATATCTGACACG |
| HindIII601 R | AAGCTTACTGGAGAATCCCGGTCTGC |
| NheI601 F | GCTAGCACAGGATGTATATATCTGACACG |
| NheI601 R | GCTAGCACTGGAGAATCCCGGTCTGC |
| NheI_5t | /5Phos/CTAGCTTTTTG |
| NheI_5a | /5Phos/CTAGCAAAAAG |
| HindIII_5t | /5Phos/AGCTTTTTTTA |
| HindIII_5a | /5Phos/AGCTTAAAAAA |
| N_R_polyT | /5Phos/CTAGCAAAAAAAAAAAAAAAAAAAAG |
| N_F_polyT | /5Phos/CTAGCTTTTTTTTTTTTTTTTTTTTG |
| H_R_polyT | /5Phos/AGCTTAAAAAAAAAAAAAAAAAAAAA |
| H_F_polyT | /5Phos/AGCTTTTTTTTTTTTTTTTTTTTTTA |
| 70R_N_pSJ322 | GCTAGCAAATTATTGGTTTTTAATGTTTTTTGCTG |
| 70F_N_pSJ322 | GCTAGCTATTTCAAAAATAATTTTTCTAGTATAATTC |
| 70R_H_pSJ322 | AAGCTTAAATTATTGGTTTTTAATGTTTTTTGCTG |
| 70F_H_pSJ322 | AAGCTTTATTTCAAAAATAATTTTTCTAGTATAATTC |
| 0SMJ023 | /5Phos/CTAGCTTTTTGTTTTTG |
| oSMJ024 | /5Phos/CTAGCAAAAACAAAAAG |
| oSMJ118 | ATGGAAAAACGCCAGCAACG |
| oSMJ119_5Biotin | TATGAGGACGGTATACATTCG |