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Effect of cell ratio on induction of TNF-α by SEB. Purified monocytes and lymphocytes were mixed in different cell ratios and plated in 24-well plates in four replicates at a density of 106 cells/well. Cells were cultured for 20 h in the presence of 10 (filled bars) and 100 (striped bars) ng of SEB per ml. Culture media were collected and assayed in duplicate for TNF-α production.
