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Dependence on concentration of SEB for TNF-α induction. Cells were plated in four replicates at a density of 106/well and then cultured for 20 h in the presence of different concentrations of SEB. Culture media were collected and assayed in duplicate for TNF-α production. (A) Monocytes (▵), lymphocytes (□), and mixed cells (○). (B) Monocytes alone in the presence of high concentrations of SEB.
