FIG. 5.

Effect of PKC inhibitors on induction of TNF-α by SEB. Monocytes and lymphocytes were plated in four replicates; incubated with either sphingosine (▵), chelerythrine (○), or H7 (□) for 60 min followed by stimulation with 100 ng of SEB per ml for 20 h; and then analyzed for TNF-α. Statistical analysis (t test) of significant differences compared to SEB alone showed P < 0.05 at the concentrations 10 and 20 μM for H7 and at 20 μM only for the other two inhibitors.