FIG. 4.

Disruption of WdCHS4 gene in W. dermatitidis. (A) Predicted structure for integration of pDCS4-800 at the wdchs4 locus. (B) Southern hybridization analysis of the wdchs4Δ-1 disruptant strain. The recombinant plasmid pDCS4-800 linearized with BstXI was transformed into W. dermatitidis 8656. Southern blots of genomic DNA from W. dermatitidis 8656 (lanes 1 and 3) and wdchs4Δ-1 strain (lanes 2 and 4), digested with ApaI (lanes 1 and 2) and SacI (lanes 3 and 4), were hybridized with a 0.8-kb WdCHS4 BamHI fragment.