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. Author manuscript; available in PMC: 2023 May 7.
Published in final edited form as: Nat Struct Mol Biol. 2022 Nov 7;29(11):1122–1135. doi: 10.1038/s41594-022-00856-x

Extended Data Fig. 4. LSD1-BAF interaction in endocrine sensitive and resistant cells.

Extended Data Fig. 4

a, Superose 6 gel filtration in parental cells showing co-elution of LSD1, RCOR1, and members of the SWI/SNF complex (SMARCC1 and ARID2). b, LSD1 IP with SWI/SNF subunits in reprogrammed T47D. Note that the DPF2-LSD1 interaction was also not detected by LC-MS/MS (Fig. 2e). c, SMARCC1 IP with LSD1 in reprogrammed T47D. d, The LSD1-SMARCC1 interaction is DNA-independent. EtBr, ethidium bromide1. e-f, Proliferation of T47D treated with 1µM corin for 5 days (e), and TSA or SAHA for 7 days (f), n = 3 biological independent replicates. Data are presented as mean values + SEM, p-value < 0.001 (two-way ANOVA). g, Proliferation of WT and LSD1 KO reprogrammed T47D treated with TSA or SAHA for 7 days, n = 2 independent experiments. h, ERα WB in parental and endocrine resistant T47D lines with VINCULIN as a loading control. i, Synergy maps of parental and TamR T47D treated with tamoxifen and corin, or fulvestrant and corin. The 3D synergy matrix was generated with SynergyFinder 2.0, n = 3 biological independent replicates. Uncropped images are available as source data.