a, Impaired proliferation of parental and reprogrammed LSD1
KO and primed siLSD1 T47D, n = 3. n = 3
biological independent replicates. Data are presented as mean values + SEM.
Significance determined by two-way ANOVA, p-value <
0.05. b, Clonogenic assay of parental and reprogrammed LSD1 KO
cells. c, Volcano plots of DEGs (FC > 1.5, adjusted
p-value < 0.05). d, GSEA suggests a
downregulation of metastatic potential and estrogen response following LSD1
loss. e, Significant LSD1 interactions assayed by endogenous
immunoprecipitation followed by LC-MS/MS (orange = LSD1/CoREST subunits, blue =
other known LSD1 interactors, red = BAF subunits), n = 3
biological independent replicates. f, GSK-LSD1 and corin
IC50 curves, n = 3 biological independent
replicates. GSK-LSD1 exhibited no inhibitory activity. Corin IC50
values: parental = 1.191µM, primed =
0.6877µM, reprogrammed =
1.071µM. Data are presented as mean values +
SEM.