a, Schematic of experimental design (n =
10/group). b-c, Quantification of primary tumors (b)
(p-value < 0.005; Two-way RM ANOVA) and metastatic
lesions (c), (p-value < 0.001; unpaired
two-tailed test). Data are presented as mean values + SD. d,
Survival probability (p-value = 0.0082, Log-rank [Mantel-Cox
test]). e-g, Quantification of parental (e),
reprogrammed (f), and T47D-FulR (g) primary tumors
(p-value < 0.001, n = 10/group,
Two-way RM ANOVA). Data are presented as mean values + SEM. Mice were treated
daily with vehicle or corin (7.5mg/kg). h, RNA-seq heatmap of 930
LSD1 target genes in patients resistant to chemo-endocrine treatment.
i, ATAC-seq signal at the 930 LSD1 targets in WT and LSD1 KO
reprogrammed T47D, and in reprogrammed cells treated with DMSO or 500nM of corin
for 72h. j, SMARCC1 ChIP-seq signal at the 930 LSD1 targets in WT
and LSD1 KO reprogrammed T47D. k, Expression of LSD1 targets in
reprogrammed T47D treated with 500nM corin for 72h, (p-value =
1.1e-7, t-test, two-sided). The box plots span from the 25th to 75th
percentiles, the center line shows the median and whiskers show maximum and
minimum values). l, Proposed model.