Figure 1.

Interleukin-13 (IL-13) causes an up-regulation of RhoA protein concurrently with a down-regulation of miR-140-3p in cultured human bronchial smooth muscle cells. Cells were treated with IL-13 (100 ng/mL) or its vehicle for 24 hours, and proteins and total RNAs including miRNAs were extracted. (a) (Upper panel) Representative Western blots. The relative expression of RhoA to glyceraldehyde 3-phosphate dehydrogenase (GAPDH) proteins in each sample was calculated and the data are summarized in lower panel. (b) miR-140-3p expression level determined by quantitative real-time reverse transcriptase-polymerase chain reaction. The relative gene expressions of miR-140-3p to U6 snRNA was calculated by the 2^−ΔΔCT methods. Results are presented as mean ± S.E. from 6 independent experiments. **p < 0.01 versus Vehicle group by unpaired Student’s t-test