Figure 3.
NS1 inhibits growth of KPC cells in vitro. A) Effects of DOX-induced NS1 expression on proliferation of KPCNS1 cells. Results at each time point represent the average of triplicate wells. B) Effect of DOX-induced NS1 expression on anchorage-independent growth of KPCNS1 cells. KPCNS1cells were plated in soft agar and grown ±DOX. C) Colony numbers from three independent wells for each condition were quantified using NIH ImageJ software. Graphs represent the average number of colonies per well ± s.d. p value was calculated by unpaired, two-tailed t test between -DOX and +DOX conditions and is indicated on the graphs.