Figure 6.
NS1 inhibition of KRAS stimulates an adaptive immune response. Tumours from KPCNS1 PDACs were immunophenotyped by immunohistochemistry for CD4+ or CD8+ (A) Images acquired from tumours in an untreated mouse, a mouse treated +DOX(D1), and a mouse treated +DOX(D5). First row: DAPI staining in blue, CD4 in yellow and CD8 in cyan. The segmented nuclei are overlaid as grey circles. The scale bar corresponds to 50 µm. Second row: segmentation masks showing identified CD4+ and CD8+ cells. B) Proportion of CD4+ and CD8+ cells for untreated, +DOX(D1), and +DOX(D5) mice. p values were calculated using one-way ANOVA with Dunnett’s multiple comparisons test comparing each condition to -DOX control.