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. 2022 Dec;28(12):2416–2424. doi: 10.3201/eid2812.220552

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Emerging Infectious Diseases is a publication of the U.S. Government. This publication is in the public domain and is therefore without copyright. All text from this work may be reprinted freely. Use of these materials should be properly cited.

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Maximum-likelihood phylogenetic tree of polyprotein sequences obtained from tick-borne encephalitis virus RNA–positive Ixodes ricinus ticks collected from 3 locations in the Netherlands during 2016–2020 (in bold). Additional published sequences obtained from GenBank are included for reference. Louping ill virus is used as the outgroup. Sample ID or GenBank accession numbers are indicated for each sequence, with location in brackets (if known) and country code, original isolation source, and collection year of each sample. Numbers next to each branch indicate the percentage of trees resulting from bootstrapping on the basis of 1,000 pseudoreplicate datasets for which the associated taxa clustered together. Scale bar represents the percentage of genetic variation along tree branches.