Table 2. Sequence and position of the AHSV-1 DIVA primer and probes used in study of development of PCR to characterize affecting strain of AHSV, Thailand* .

Primer/probe	Sequence, 5′ → 3′	Sense	Position
VP5-DIVA-F	5′ AGCGTCGGATGCAAAGAAATC 3′	+	1335–1355
VP5-DIVA-P1	5′ FAM- ACTACAGCTGGTGCATAAC 3′ MGB	+	1426–1444
VP5-DIVA-P2-vac	5′ FAM- TTTACAGTTGGTTCATAAT 3′ MGB	+	1426–1444
VP5-DIVA-R	5′ AAGCGCGTTCATTATCGTCC 3′	–	1494–1513

*Position numbering is with reference to AHSV-1 (GenBank accession no. KT030334). AHSV, African horse sickness virus; DIVA, Differentiating Infected from Vaccinated Animals.