Table 4. Comparison of assay sensitivity among different AHSV assays* .

Sample	Method	Limit of detection	AUC	Slope
AHSV-1 Thailand 110983/63	AHSV VP7 rRT-PCR	[10−5]	0.943	−3.89
	AHSV-1 VP2 Serotyping rRT-PCR	[10−4]
	AHSV-1 VP5 DIVA rRT-PCR 1	[10−4]
AHSV-1 OBP LAV	AHSV VP7 rRT-PCR	[10−6]	0.900	−3.49
	AHSV-1 VP2 Serotyping rRT-PCR	[10−5]
	AHSV-1 VP5 DIVA rRT-PCR 2	[10−5]

*The limit of detection was reported from the lowest observed amount of DNA amplified below the Ct <40 threshold. The AUC and slope values derived from the calibration curve are also listed. AHSV, African horse sickness virus; AUC, area under the receiver operating curve; Ct, cycle threshold; DIVA, Differentiating Infected from Vaccinated Animals; OBP LAV, Onderstepoort Biologic Products (https://www.obpvaccines.co.za) live attenuated vaccine; rRT-PCR, real-time reverse transcription PCR; VP, viral protein.