Table. Detection results for MPXV by real-time reverse transcription PCR in clinical specimen types submitted to the Public Health Ontario Laboratory, Toronto, Ontario, Canada*.
| Specimen types and number | Blood, n = 190 | Nasal/NP, n = 137 | Throat/OP, n = 106 | Skin lesions, n = 559 | Urine, n = 41 |
|---|---|---|---|---|---|
| Positive |
29 (15.3) |
20 (14.6) |
31 (29.2) |
177 (31.7) |
6/41 (14.6) |
| Specimens from positive patients |
29/67 (43.3) |
20/36 (55.6) |
31/46 (67.4) |
177/213 (83.1) |
6/21 (28.6) |
| Target | |||||
| Orthopoxvirus | |||||
| No. positive | 26 | 16 | 24 | 135 | 5 |
| Mean Ct (SD), range | 38.6 (4.1), 27.2‒40.0 | 36.3 (6.3), 23.3‒38.8 | 32.0 (6.5), 17.5‒38.0 | 27.1 (7.3), 14.3‒ 39.6 | 37.3 (5.1), 29.0‒37.9 |
| Monkeypox | |||||
| No. positive | 15 | 11 | 13 | 74 | 4 |
| Mean Ct (SD), range | 35.9 (2.1), 32.2‒37.9 | 32.4 (5.6), 18.2‒37.7 | 27.8 (5.1), 19.2‒36.0 | 23.1 (6.5), 12.0‒37.9 | 32.2 (5.5), 27.2‒37.7 |
| Clade II | |||||
| No. positive | 15 | 13 | 13 | 75 | 4 |
| Mean Ct (SD), range | 35.3 (2.3), 31.2‒37.1 | 32.8 (5), 18.1‒37.6 | 27.3 (4.5), 19.6‒35.2 | 23.1 (6.6), 11.1‒37.4 | 32.2 (5.1), 27.7‒ 37.2 |
*Values are no. (%) except as indicated. Among 30 additional specimens not shown, MPXV was detected in 2/5 semen, 1/4 saliva, 0/1 cerebrospinal fluid, and none of 20 specimens with undocumented sources. Indeterminate results are not included; those are blood 6/190 (3.2%), nasal/NP 5/137 (3.6%), throat/OP 2/106 (1.9%), skin lesion 10/559 (1.8%), and urine 6/41 (14.6%). Ct, cycle threshold; MPXV, monkeypox virus; NP, nasopharyngeal; OP, oropharyngeal.