FIG. 2.

Immunolocalization of CaGC in adult female B. malayi parasites exposed to 25 nM recombinant human CaGC (A and C) or medium without CaGC (B) for 20 min. After exposure, the adult parasites were fixed, processed for plastic embedding, sectioned, incubated with a rat anti-human CaG, and counterstained with hematoxylin and eosin as outlined in Materials and Methods. Immunostaining was restricted to the hypodermis-lateral chord. Additional negative controls included incubation of tissue sections from test and control parasites with normal rat serum, an irrelevant antibody (rat anti-human serum albumin), or no antibody (conjugate control). The results from all of the negative control reactions were negative (data not shown). U, uterus.