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Experimental system for multiplex evaluation of wild-type Htt and Hs HTT mRNA subcellular localization in brain slices. (A–D) Dual-colour FISH probe sets targeting: mouse (Mm) Htt mRNA in exons 27–35, human (Hs) HTT mRNA in exons 29–36 and Mm Hprt mRNA in exons 1–9 in (A) YAC128, (B) B97-ΔN17, (C) wild-type and (D) B31-ΔN17 mice. (E) Regions of the mouse brain used for RNAscope image analysis.
