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. 2022 Oct 13;4(6):fcac248. doi: 10.1093/braincomms/fcac248

Figure 3.

Figure 3

Repeat expansion increases nuclear retention of mutant Hs HTT mRNA and forms clusters in B97-ΔN17 mouse striatum. (A) Mm Htt and Hs HTT mRNAs were detected in B97-ΔN17 mouse striatum by FISH. Nuclei labelled with Hoechst. Scale bar, 5 µm. (B) Percentage of nuclear Mm Htt and Hs HTT mRNAs in wild-type (WT) and B97-ΔN17 mice at 1, 3, 6, and 9 months old (n = ∼100 cells per brain region pooled from three mice). (C) Scatter plot showing the volume of individual mRNA foci or clusters (see Methods for volume calculation). Each point represents the volume of individual mRNA foci. (D) Cumulative frequency distribution plot of RNA foci volume. The yellow shaded area represents the cut-off for a cluster, which is defined as ≥0.6 µm3. The thick line represents the mean. (E) Percentage of cells containing Mm Htt or Hs HTT mRNA clusters in B97-ΔN17 mouse striatum (n = ∼100 cells per brain region pooled from three mice, each point represents a mouse). For all panels, ns = not significant, **P < 0.01, ***P < 0.001, ****P < 0.0001, one-way ANOVA, Bonferroni’s multiple comparisons test.