Fig 6. The in vivo expression of TsCRT+TsSP1.1, TsCRT and TsSP1.1 in spleen and MLN of immunized mice were assessed using IIFT.

IIFT with anti-rTsCRT and anti-rTsSP1.1 sera showed that immunostaining was observes in vaccinated murine spleen (A) and MLN (B) sections, but not in spleen and MLN sections probed using normal serum. No immunostaining in spleen and MLN sections of mice inoculated with only empty pcDNA3.1 and PBS alone was detected by immune serum. Scale bar: 200 μm.