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Levels of total sIgA (A), Trichinella-specific sIgA (B) and histamine (C) in gut washes of vaccinated mice. The data are shown as the mean OD values ± SD for five mice per group. No notable specific sIgA response was detected in the pcDNA3.1 and PBS control groups. The vaccination times are shown with arrows (↑) and the challenge time is indicated by triangles (△). *P < 0.0001 compared with PBS group. #P < 0.001 compared among various immunization groups.
