Fig 9. Cytokines secreted by spleen, mesenteric lymph nodes (MLN) and Peyer’s patches (PP) from mice immunized with TsCRT+TsSP1.1, TsCRT or TsSP1.1 at different times after immunization.

Concentrations of two cytokines (IFN-γ and IL-4) were measured in supernatant after the spleen, MLN and PP cells were stimulated with 2 μg of ML soluble crude antigens for 72 h at 37°C and 5% CO₂. The data are shown as the mean ± SD of five mice per group. The vaccination times are shown with arrows (↑) and the challenge time is indicated by triangles (Δ). * P < 0.001 compared to the PBS control groups. ^#P < 0.05 compared among various vaccination groups.