Genesis of the transforming plasmid pCPW7, in which the
PEP1 (SAP1) ORF is under the regulation of the
WH11 promoter. (A) The endogenous ADE2 gene with
relevant restriction sites. (B) Plasmid pCRW5, which was used to
construct pCPW7. (C) The final transforming plasmid pCPW7.
ADE2, the adenine 2 gene of C. albicans WO-1;
RLUC, the R. reniformis luciferase ORF; WH11 5′,
the WH11 promoter sequence; Ampr, ampicillin
resistance gene; PEP1, the PEP1 (SAP1)
ORF.