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. 1999 Dec;67(12):6652–6662. doi: 10.1128/iai.67.12.6652-6662.1999

FIG. 1.

FIG. 1

Genesis of the transforming plasmid pCPW7, in which the PEP1 (SAP1) ORF is under the regulation of the WH11 promoter. (A) The endogenous ADE2 gene with relevant restriction sites. (B) Plasmid pCRW5, which was used to construct pCPW7. (C) The final transforming plasmid pCPW7. ADE2, the adenine 2 gene of C. albicans WO-1; RLUC, the R. reniformis luciferase ORF; WH11 5′, the WH11 promoter sequence; Ampr, ampicillin resistance gene; PEP1, the PEP1 (SAP1) ORF.