TABLE 1.
Misexpression of PEP1 (SAP1) in the white phase does not affect the apparent frequencies of switching in the white to opaque phase or opaque to white phasea
Strain | Original population phenotypeb | No. of colonies analyzeda | Proportion of opposite-phase phenotypea |
---|---|---|---|
EPB 3/15 | White | 8,333 | 1.1 × 10−3 |
Opaque | 2,552 | 1.5 × 10−3 | |
CPW 5/3 | White | 2,174 | 0.9 × 10−3 |
Opaque | Not tested | ||
CPW 7/178 | White | 5,130 | 2.7 × 10−3 |
Opaque | 3,957 | 5.0 × 10−4 |
Switching frequencies are reflected by the proportion of CFU of the opposite phenotype in a clonal colony.
Cells from a colony exhibiting one phenotype (e.g., white) were in turn clonally plated, and the proportion of colonies with the opposite-phase phenotype was measured.