Table 1. Oocyte and nurse cell (NC) gene expression.
| Gene | Avg E14.5 | E14.5 NC | E14.5 NC/Avg | Avg 16.5 | E16.5 NC | E16.5 NC/Avg | Avg 18.5 | E18.5 NC | E18.5 NC/Avg | P1 O | P1 NC | P1 NC/P1 O | P5 O |
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| Oocyte | |||||||||||||
| Figla | 30.60 | 10.30 | 0.34 | 47.19 | 45.56 | 0.97 | 147.10 | 132.35 | 0.90 | 204.59 | 94.24 | 0.46 | 63.14 |
| Nobox | 0.21 | 0.00 | 0.00 | 3.42 | 5.03 | 1.47 | 32.27 | 26.75 | 0.83 | 74.70 | 24.97 | 0.33 | 31.92 |
| Padi6 | 0.21 | 0.00 | 0.00 | 0.00 | 0.00 | nd | 0.45 | 0.00 | 0.00 | 31.76 | 4.87 | 0.15 | 69.97 |
| Filia/Khdc3 | 0.76 | 0.00 | 0.00 | 0.22 | 0.00 | 0.00 | 0.97 | 0.00 | 0.00 | 2.50 | 0.00 | 0.00 | 16.14 |
| Floped/Ooep | 11.64 | 8.32 | 0.71 | 3.22 | 0.00 | 0.00 | 16.40 | 12.11 | 0.74 | 57.59 | 25.07 | 0.44 | 80.86 |
| Nlrp5/MATER | 0.31 | 0.00 | 0.00 | 0.41 | 2.61 | 6.37 | 1.69 | 3.46 | 2.05 | 31.72 | 17.71 | 0.56 | 30.17 |
| Tle6 | 5.16 | 4.26 | 0.83 | 6.27 | 0.00 | 0.00 | 28.24 | 18.47 | 0.65 | 75.58 | 32.94 | 0.44 | 59.98 |
| E14.5 NC | |||||||||||||
| Fst | 4.75 | 19.05 | 4.01 | 2.16 | 2.13 | 0.99 | 8.00 | 4.31 | 0.54 | 1.90 | 3.89 | 2.05 | 1.52 |
| Tex14 | 79.30 | 24.30 | 0.31 | 58.70 | 15.20 | 0.26 | 34.10 | 18.70 | 0.55 | 26.00 | 3.10 | 0.12 | 9.44 |
| Pard6b | 3.34 | 10.51 | 3.15 | 4.15 | 0.00 | 0.00 | 4.22 | 0.89 | 0.21 | 9.72 | 0.33 | 0.03 | 1.81 |
| Casp8 | 0.46 | 3.81 | 8.29 | 1.82 | 4.85 | 2.67 | 0.36 | 0.73 | 2.00 | 0.32 | 0.00 | 0.00 | 0.62 |
| Wt1 | 4.04 | 12.60 | 3.12 | 3.74 | 4.05 | 1.08 | 6.58 | 10.79 | 1.64 | 5.18 | 3.01 | 0.58 | 2.42 |
| Meis2 | 2.05 | 6.67 | 3.24 | 2.39 | 6.24 | 2.61 | 1.95 | 1.72 | 0.88 | 3.86 | 1.14 | 0.30 | 1.84 |
| Kitl | 3.49 | 14.92 | 4.27 | 6.17 | 13.98 | 2.26 | 10.05 | 5.95 | 0.59 | 5.03 | 0.00 | 0.00 | 0.09 |
| Calb1 | 0.51 | 4.69 | 9.17 | 1.38 | 3.60 | 2.60 | 0.53 | 2.31 | 4.34 | 0.38 | 0.00 | 0.00 | 0.30 |
| E16.5 NC | |||||||||||||
| Fstl1 | 6.51 | 7.71 | 1.18 | 4.00 | 12.00 | 3.00 | 3.77 | 2.07 | 0.55 | 4.69 | 3.20 | 0.00 | 1.71 |
| Cdkn1b | 4.08 | 11.85 | 2.92 | 5.96 | 13.47 | 2.26 | 2.70 | 0.00 | 0.00 | 1.94 | 0.00 | 0.00 | 7.91 |
| Stmn3 | 0.60 | 4.05 | 6.74 | 0.20 | 1.66 | 8.30 | 0.15 | 1.30 | 8.87 | 0.00 | 0.00 | nd | 0.00 |
| Jmjd8 | 1.34 | 4.84 | 3.62 | 0.81 | 2.61 | 3.23 | 3.20 | 3.46 | 1.08 | 2.61 | 1.38 | 0.53 | 1.60 |
| Igfbp5 | 3.62 | 17.54 | 4.84 | 13.83 | 35.70 | 2.58 | 10.23 | 2.86 | 0.28 | 5.83 | 1.18 | 0.20 | 4.23 |
| E18.5 NC | |||||||||||||
| Rspo1 | 2.28 | 2.13 | 0.94 | 0.49 | 2.61 | 5.29 | 1.16 | 2.65 | 2.28 | 2.56 | 1.34 | 0.45 | 0.13 |
| Serpinb6b | 0.51 | 2.25 | 4.41 | 0.57 | 1.04 | 1.82 | 0.60 | 2.18 | 3.64 | 0.55 | 0.00 | 0.00 | 0.41 |
| P1 NC | |||||||||||||
| Cgn | 3.80 | 6.17 | 1.62 | 1.59 | 2.75 | 1.73 | 2.01 | 0.00 | 0.00 | 0.99 | 3.10 | 3.13 | 1.98 |
| Tmod1 | 0.74 | 7.32 | 9.87 | 2.67 | 0.00 | 0.00 | 1.30 | 0.00 | 0.00 | 0.66 | 1.97 | 2.99 | 0.14 |
| Abhd2 | 5.13 | 9.23 | 1.80 | 1.06 | 2.23 | 2.11 | 1.72 | 0.72 | 0.42 | 0.18 | 2.37 | 12.76 | 0.58 |
| Dusp3 | 0.13 | 3.28 | 24.37 | 0.33 | 1.64 | 4.90 | 0.71 | 0.00 | 0.00 | 0.60 | 2.29 | 3.79 | 1.04 |
Legend. Mouse ovary gene expression of the indicated genes at the indicate times in mixed cells (unactivated NC and oocytes, indicated by ‘Avg’), activated NCs or oocytes (O) were determined by single-cell RNA sequencing (scRNAseq; data from Niu and Spradling, 2022). Genes are grouped into those thought to function in oocytes (top section) and in lower sections, those thought to function in nurse cells peaking at the indicated times (i.e. E14.5 NC). Possible function of genes in bold are discussed in the text. E14.5=embryonic day 14.5, etc.; P1=postnatal day 1, etc.; ratios (NC/Avg) from the previous two columns are shown in italics. See text for discussion of specific genes. nd = not done.