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. 2022 Nov 2;11:e80956. doi: 10.7554/eLife.80956

Figure 5. Keap1-Nrf2 signaling drives invasiveness of Lgl-KD multilayers.

(A) Representative confocal images of ovarioles containing egg chambers with transgene expression in follicle cells (green) driving Lgl-KD (top), Lgl-KD+Cnc-KD (middle), and Lgl-KD+Keap1-KD (bottom panels). Degenerating egg chambers are marked by asterisks (*). Border cell migration defects are indicated by arrowheads. Nucleus is marked by DAPI (white). F-actin is marked by Phalloidin (red). Scale bars: 20 µm. (B) Box-and-whisker plot showing the quantification of phenotypic rescue at early (red), mid (blue), and late (green) oogenesis. For the multilayering phenotype at midoogenesis, we have only counted instances of >2 follicular layers. (C) Bar plot showing relative expression levels of genes involved in the Keap1-Nrf2 signaling pathway in relevant genotypes (N = minimum 10 pairs of ovaries). Samples are color-coded as shown in legend and the error bars represent Standard Error (SE). (D) Confocal images of ovarioles with Lgl-KD+Keap1-OE (above) and Lgl-KD+CncC-OE (below) in follicle cells (green). Arrowheads mark the epithelial bridging or fusion phenotype. Nucleus is marked by DAPI (white). F-actin is marked by Phalloidin (red). Scale bars, 20 µm.

Figure 5—source data 1. Quantification of the rescue of stage-specific Lgl-KD phenotypes upon Cnc-KD and Keap1-KD.
Related to Figure 5B.

Figure 5.

Figure 5—figure supplement 1. Epithelial multilayering and invasiveness are increased in Lgl-KD+CncC-OE egg chambers.

Figure 5—figure supplement 1.

(A) Confocal images showing increased invasiveness of the epithelia containing Lgl-KD+CncC-OE follicle cells (green). Nurse cell (yellow arrow) is depicted being compressed by invading Lgl-KD+CncC-OE epithelia (marked by white arrowheads). (B) Confocal images comparing mid-stage egg chambers containing Lgl-KD (top panels) and Lgl-KD+CncC-OE (bottom panels) follicle cells, 2 days following heat shock induction of transgene expression. Enhancement of multilayer formation in Lgl-KD+CncC-OE egg chambers at midoogenesis is observed in 22.85% ovarioles (N = 16/70) compared to 2% ovarioles (N = 50) containing Lgl-KD egg chambers. Nucleus is marked by DAPI (white), and F-actin is marked by Phalloidin (red). Scale bars, 20 µm.