Appendix 1—key resources table.
| Reagent type (species) or resource | Designation | Source or reference | Identifiers | Additional information |
|---|---|---|---|---|
| Genetic reagent (Cryptococcus neoformans KN99α, matα) | WT strain: H99 | Nielsen et al., 2003 | ||
| Genetic reagent (C. neoformans KN99α, matα) | WT strain: H99a | Nielsen et al., 2003 | ||
| Genetic reagent (C. neoformans KN99α, matα) | cbk1Δ | Walton et al., 2006 | FJW9 | H99alpha, CBK1::NATr |
| Genetic reagent (C. neoformans KN99α, matα) | mob2Δ | Walton et al., 2006 | FJW10 | H99alpha, MOB2::NATr |
| Genetic reagent (C. neoformans KN99α, matα) | kic1Δ | Walton et al., 2006 | FJW8 | H99alpha, KIC1::NATr |
| Genetic reagent (C. neoformans KN99α, matα) | tao3Δ | Walton et al., 2006 | AI136 | MATalpha, TAO3:: NATr |
| Genetic reagent (C. neoformans KN99α, matα) | sog2Δ | Walton et al., 2006 | AI131 | MATalpha, SOG2:: NATr |
| Genetic Reagent (Candida albicans, SN250) | SN250 | Wakade et al., 2020 | SN250 | HIS-, LEU-, ARG- |
| Genetic Reagent (C. albicans, SN250) | cbk1ΔΔ | Wakade et al., 2020 | ΔΔcbk1 | SN250, ΔΔcbk1: HIS-, LEU-, ARG- |
| Genetic reagent (C. neoformans KN99α, matα) | cna1Δ | FGSC deletion set Plate 46 Well E12 | cna1Δ | H99alpha CNA1::NATr |
| Genetic reagent (C. neoformans KN99α, matα) | cdc24Δ | FGSC deletion set Plate 33 Well C4 | cdc24Δ | H99alpha, CDC24::NATr |
| Genetic reagent (C. neoformans KN99α, matα) | mpk1Δ | FGSC deletion set Plate 11 Well A5 | mpk1Δ | H99alpha, MPK1::NATr |
| Genetic reagent (C. neoformans KN99α, matα) | CBK1 OE | This study. | BC1449 | H99alpha, PCTR4-CBK1-mCherry- NEOr |
| Genetic reagent (C. neoformans KN99α, matα) | cdc24Δ, CBK1OE | This study. | BC1281 | H99alpha, CDC24::NATr, PCTR4-CBK1-mCherry- NEOr |
| Genetic reagent (C. neoformans KN99α, matα) | cdc24Δ, CBK1OE | This study. | BC1282 | H99alpha, CDC24::NATr, PCTR4-CBK1-mCherry- NEOr |
| Genetic reagent (C. neoformans KN99α, matα) | cna1Δ, CBK1OE | This study. | BC1283 | H99alpha, CNA1::NATr, PCTR4-CBK1-mCherry- NEOr |
| Genetic reagent (C. neoformans KN99α, matα) | cna1Δ, CBK1OE | This study. | BC1284 | H99alpha, CNA1::NATr, PCTR4-CBK1-mCherry- NEOr |
| Genetic reagent (C. neoformans KN99α, matα) | mpk1Δ, CBK1OE | This study. | BC1285 | H99alpha, MPK1::NATr, PCTR4-CBK1-mCherry- NEOr |
| Genetic reagent (C. neoformans KN99α, matα) | mpk1Δ, CBK1OE | This study. | BC1286 | H99alpha, MPK1::NATr, PCTR4-CBK1-mCherry- NEOr |
| Genetic reagent (C. neoformans KN99α, matα) | cdc24Δ, CDC24OE | This study. | BC650 | H99alpha, CDC24::NATr, PCTR4-mNeonGreen-CDC24-NEOr |
| Genetic reagent (C. neoformans KN99α, matα) | cbk1Δ, CBK1OE | This study. | BC669 | H99alpha, CBK1::NATr, PCTR4-CBK1-mCherry- NEOr |
| Genetic reagent (C. neoformans KN99α, matα) | mpk1Δ, MPK1OE | This study. | BC1356 | H99alpha, MPK1::NATr, PGPD1-mNeonGreen-MPK1-NEOr |
| Genetic reagent (C. neoformans KN99α, matα) | cna1Δ, CNA1OE | Kozubowski et al., 2011 | LK214 | H99a, CNA1::NEOr, PH3-GFP-CNA1-NATr |
| Genetic reagent (C. neoformans KN99α, matα) | cbk1Δ, CDC24OE | This study. | BC1357 | H99alpha, CBK1::NATr, PCTR4-mNeonGreen-CDC24-NEOr |
| Genetic reagent (C. neoformans KN99α, matα) | cbk1Δ, MPK1OE | This study. | BC1358 | H99alpha, CBK1::NATr, PGPD1-mNeonGreen-MPK1-NEOr |
| Genetic reagent (C. neoformans KN99α, matα) | cbk1Δ, CNA1OE | This study. | BC1359 | H99alpha, CBK1::NATr, PH3-GFP-CNA1-NATr |
| Genetic reagent (C. neoformans KN99α, matα) | sup1 | This study. | BC1068 | H99alpha, CBK1::NATr,SUP1 |
| Genetic reagent (C. neoformans KN99α, matα) | sup2 | This study. | BC1076 | H99alpha, CBK1::NATr,SUP2 |
| Genetic reagent (C. neoformans KN99α, matα) | cbk1Δssd1Δ | This study. | BC1239 | H99alpha, CBK1::NATr, SSD1::NEOr |
| Genetic reagent (C. neoformans KN99α, matα) | ssd1Δ | This study. | BC1241 | H99alpha, SSD1::NEOr |
| Genetic reagent (C. neoformans KN99α, matα) | cbk1Δpsc1Δ | This study. | BC1369 | H99alpha, CBK1::NATr, PSC1::HYGr |
| Genetic reagent (C. neoformans KN99α, matα) | psc1Δ | This study. | BC1393 | H99alpha, PSC1::HYGr |
| Genetic reagent (C. neoformans KN99α, matα) | cac1Δ | Bahn et al., 2006 | YSB42 | H99alpha, CAC1::NATr STM#159 |
| Genetic reagent (C. neoformans KN99α, matα) | aca1Δ | FGSC deletion set Plate 32 Well H6 | aca1Δ | H99alpha, ACA1::NATr |
| Genetic reagent (C. neoformans KN99α, matα) | pkr1Δ | FGSC deletion set Plate 33 Well H7 | pkr1Δ | H99alpha, PKR1::NATr |
| Genetic reagent (C. neoformans KN99α, matα) | pde1Δ | FGSC deletion set Plate 10 Well A12 | pde1Δ | H99alpha, PDR1::NATr |
| Genetic reagent (C. neoformans KN99α, matα) | pde2Δ | FGSC deletion set Plate 11 Well H7 | pde2Δ | H99alpha, PDR2::NATr |
| Genetic reagent (C. neoformans KN99α, matα) | gpa1Δ | Bahn et al., 2005 | YSB83 | H99alpha, GPA1::NATr STM#5 |
| Genetic reagent (C. neoformans KN99α, matα) | cln1Δ | This study. | BZ36 | H99alpha, CLN1::NATr |
| Genetic reagent (C. neoformans KN99α, matα) | ecm2201Δ | FGSC deletion set Plate 2 Well A10 | ecm2201Δ | H99alpha, ECM2201::NATr |
| Genetic reagent (C. neoformans KN99α, matα) | kin4Δ | Bahn Kinase Deletion Set Plate 4 Well G8 (Lee et al., 2016) | kin4Δ | H99alpha, KIN4::NATr |
| Recombinant DNA reagent | pPZP-NATcc | Dickinson et al., 2013 | pPZP-NATcc | |
| Recombinant DNA reagent | pDD162 | Walton et al., 2006 | pDD162 | |
| Recombinant DNA reagent | pFZ1-CDC24 | This study. | PCTR4-2-mNeonGreen-CDC24(H99)-NEOr | |
| Recombinant DNA reagent | pXC-CBK1-mCh | This study. | PCTR4-2-CDC24(H99)-mCherry-NEOr | |
| Recombinant DNA reagent | LKB61 | Kozubowski et al., 2011 | PGPD1-mCherry-CNA1-HYGr | |
| Recombinant DNA reagent | pUC19-MPK1-mNG | This study. | PGPD1-MPK1-mNeonGreen-NEOr | |
| Cell line (Mus. Musculus, macrophage cell line J774A.1) |
J774A.1 | American Type Culture Collection | ATCC TIB-67 | |
| Chemical Compound and drug | Hygromycin | Research Products International | Cat. NO.: H75000 | |
| Chemical Compound and drug | G418 | Research Products International | Cat. NO.: G64000 | |
| Chemical Compound and drug | Nourseothricin | Jena Bioscience | Cat. NO.: AB-102–25 G | |
| Software and algorithm | Graphpad Prism 9 | Graphpad | ||
| Software and algorithm | nSolver software version 4.0 | NanoString Technologies | ||
| Software and algorithm | Trim Galore v0.6.5 | Krueger, 2021 | ||
| Software and algorithm | BWA aligner v0.7.17 | Li, 2013 | ||
| Software and algorithm | SAMtools v1.10 | Li et al., 2009a | ||
| Software and algorithm | Picard Tools v2.16.0 | Broad_Institute, 2022 | ||
| Software and algorithm | bcftools v1.13 | Danecek et al., 2021 | ||
| Software and algorithm | Illustrator of biological sequences (IBS) | Liu et al., 2015 |