DAY 1 (3.5 h total):	Preparation of DBCO tagged DS and dye conjugated IS 10 min of hands-on time for mixing of the reactions 3 h incubation time
DAY2 (1 h total):	45 min hands-on time for ethanol precipitation (DS-DBCO and IS-dye)
DAY3 (5.5 h total):	45 min hands-on time for ethanol precipitation (DS-DBCO and IS-dye) 1 h hands-on time for preparation of azide-tagged antibody 3 h incubation time 30 min hands-on time for determining the concentration of each reagent
DAY 4 (3 h total):	40 min hands-on time to harvest final antibody-PEG8-DS conjugates 2 h incubation time 20 min hands-on time to etch coverglass for imaging experiments
DAY 5 (1 h total):	30 min hands-on time to seed cells
DAY 6 (8 h total):	7–8 h hands-on time to perform immunostaining
Day 7 (8 h total):	Variable hands-on time for imaging and start of data processing
Day 8 (4 h total):	Variable hands-on time for data processing