Table 2.
Assignment of ions obtained by MALDI TOF MS1 screening of brain tumoral lipid mixture.
| No. | m/z | Ion | Structure |
|---|---|---|---|
| 1 | 390.948 | [M-H]− | FA 26 : 2 (17,20) or FA 26 : 2 (5,9) |
| 2 | 521.072 | [M-H]− | Cer (d18:1/15 : 0)↔Cer 33 : 1; O2 |
| 3 | 536.072 | [M-H]− | Cer (d18:1/16 : 0) or Cer (d18:0/16 : 1) or Cer (d20 : 1/14 : 0) ↔ Cer 34 : 1; O2 |
| 4 | 554.045 | [M-H]− | Cer (t18:0/16 : 0)↔Cer 34 : 0; O3 |
| 5 | 568.019 | [M-H]− | Cer (t18:0/17 : 0)↔Cer 35 : 0; O3 |
| 6 | 590.004 | [M-H]− | Cer (t18:0/19 : 3)↔Cer 37 : 3; O3 |
| 7 | 606.290 | [M-H]− | Cer (d18:1/21 : 0) or Cer (d16:1/23 : 0)↔Cer 39 : 1; O2 |
| 8 | 618.100 | [M-H]− | Cer (d18:2/22 : 0)↔Cer 40 : 2; O2 or Cer (d18:0/16 : 0)–1-P↔CerP 34 : 0; O2 |
| 9 | 640.134 | [M-H]− | Cer (t18:0/h21 : 0)↔Cer 39 : 0; O4 |
| 10 | 654.127 | [M-H]− | Cer (t18:0/24 : 6)↔Cer 42 : 6; O3 |
| 11 | 658.152 | [M-H]− | Cer (t18:0/24 : 4)↔Cer 42 : 4; O3 |
| 12 | 666.217 | [M-H]− | Cer (t18:0/24 : 0) or Cer (t20:0/22 : 0) or Cer (d20:0/h22 : 0)↔Cer 42 : 0; O3 |
| 13 | 670.121 | [M-H]− | Cer (t18 : 0/25 : 5)↔Cer 43 : 5; O3 |
| 14 | 672.144 | [M-H]− | Cer (t18:0/25 : 4)↔Cer 43 : 4; O3 |
| 15 | 692.135 | [M-H]− | Cer (d18:0/27 : 0)↔Cer 45 : 0; O2 or Cer (d18:1/h26 : 0) ↔ Cer 43 : 1; O3 |
| 16 | 714.123 | [M-H]− | Hex-Cer (t18:2/h16 : 0) |
| 17 | 728.120 | [M-H]− | SM(d18:1/18 : 1) or SM(d18 : 0/18 : 2) or SM(d18:2/18 : 0) |
| 18 | 730.094 | [M-H]− | Hex-Cer (t18 : 0/17 : 0) |
| 19 | 744.093 | [M-H]− | Hex-Cer (t18:0/18:0) or hex-Cer (d20:0/h16 : 0) |
| 20 | 762.682∗ | [M-H]− | Hex-Cer(d18:1/21 : 3) |
| 21 | 766.078 | [M-H]− | Sulfatides: HSO3-3Galβ-Cer (d18 : 1/h14:0) or Hex-Cer (d18:2/21:0) |
| 22 | 776.036 | [M-H]− | Cer (d18:1/h32 : 0)↔Cer 50 : 1; O3 |
| 23 | 792.620 | [M + Na+ − 2H+]− | Hex-Cer (d18:1/h20:0) |
| 24 | 798.594 | [M-H]− | Hex-Cer (d18:1/24 : 6) |
| 25 | 806.184 | [M-H]− | Hex-Cer (t18:0/23 : 4) |
| 26 | 864.709 | [M + K+ − 2H+]− [M-H]− |
Hex-Cer (t18:0/24 : 1) O-ac-lac-Cer (d18:1/12 : 0) |
| 27 | 886.630∗ | [M-H]− | Lac-Cer (d18:1/18 : 1) |
| 28 | 914.250 | [M-H]− | Lac-Cer (d18:1/20 : 1) |
| 29 | 921.595∗ | [M-H]− | GM4 (d16 : 0/13 : 0) |
| 30 | 953.470 | [M + 2Na − 3H]− | GM4 (d18:0/10 : 0) |
| 31 | 974.073 | [M-H]− | Sulfatides: HSO3-3Galβ-Cer (d18:1/30 : 0) Or lac-Cer (d18:0/24 : 0) |
| 32 | 1007.031 | [M-H]− | GA2 (d18:1/12 : 0) |
| 33 | 1077.730 | [M-H]− | GA2 (t18:1/16:1) |
| 34 | 1108.151 | [M-H]− | GA2 (d18:1/h18:0) |
| 35 | 1129.836 | [M-H]− | GM4(d18:1/26 : 0) |
| 36 | 1181.756 | [M-H]− | GM3 (d18:0/18 : 0) |
| 37 | 1215.049 | [M-H]− | GM4(d18:0/32:0) |
| 38 | 1407.710 | [M-H]− | KDN-GM1(t16:1/12 : 0) |
| 39 | 1426.274 | [M-H]− | GalNAcβ1-4(NeuGcα2-3)Galβ1-4Glcβ-Cer(d18:1/20 : 0) |
| 40 | 1466.801 | [M + Na − 2H]− | GD3 (d18:0/16 : 0) |
| 41 | 1467.243 | [M-H]− | GM2 (d18:1/24 : 0) |
| 42 | 1516.941 | [M-H]− | GM1 (d18:1/16 : 0) |
| 43 | 1544.947 | [M-H]− | GM1 (d18:1/18:0) |
| 44. | 1591.939 | [M-H]− | Ganglio:Galβ1-4GalNAcβ1-3Galβ1-3GalNAcβ1-4Galβ1-4Glcβ-Cer(d18:1/16:0) (Ganglio series) Lacto:Galβ1-3GlcNAcβ1-3Galβ1-3GlcNAcβ1-3Galβ1-4Glcβ-Cer (d18:1/16 : 0) (lacto series) Neolacto: Galβ1-4GlcNAcβ1-3Galβ1-4GlcNAcβ1-3Galβ1-4Glcβ-Cer (d18:1/16 : 0)(Neolacto series) Globo: GalNAcα1-3Galβ1-3GalNAcβ1-3Galα1-4Galβ1-4Glcβ-Cer (d18:1/16 : 0)(Globo series) Isoglobo: Galα1-3(GalNAcβ1-4Galβ1-4GlcNAcβ1-6) Galβ1-4Glcβ-Cer (d18:1/16 : 0)(Isoglobo series) |
| 45. | 1631.857 | [M-H]− | Type I A antigen: GalNAcα1-3(Fucα1-2)Galβ1-3GlcNAcβ1-3Galβ1-4Glcβ-Cer(d18:1/20 : 0)(lacto series) Type II A antigen: GalNAcβ1-3(Fucα1-2)Galβ1-4GlcNAcβ1-3Galβ1-4Glcβ-Cer(d18:1/20 : 0) Or GalNAcα1-3(Fucα1-2)Galβ1-4GlcNAcβ1-3Galβ1-4Glcβ-Cer(d18:1/20 : 0)(Neolacto series) Globo:Fucα2-3GlcNAcβ1-6GalNAcβ1-3Galα1-4Galβ1-4Glcβ-Cer(d18:1/20 : 0) (Globo series) Isoglobo: Fucα1-3GlcNAcβ1-3Galα1-3(GalNAcβ1-4) Galβ1-4Glcβ-Cer(d18:1/20 : 0)(Isoglobo series) |
| 46. | 1758.206 | [M-H]− | GD2(d18:1/24 : 0) |
| 47. | 1759.163 | [M-H]− | KDNα2-3Galβ1-4(Fucα1-3)GlcNAcβ1-3Galβ1-4Glcβ-Cer(d18:1/26 : 1) |
| 48. | 1873.273 | [M-H]− | GT3(d18:1/26 : 0) |
| 49. | 2092.106 | [M-H]− | Fuc-GD1b(d18:1/26 : 1) or Disialyl lea (d18:1/26 : 1): NeuAcα2-3Galβ1-3 (NeuAcα2-6) (Fucα1-4) GlcNAcβ1-3Galβ1-4Glcβ-Cer(d18:1/26 : 1) |
| 50. | 2194.225 | [M-H]− | GalNAcβ1-4(NeuAcα2-3)Galβ1-3GlcNAcβ1-3(Galβ1-3GalNAcβ1-4)Galβ1-4Glcβ-Cer(d18:1/24 : 1) |
| 51. | 2286.826 | [M-H]− | sLea -Lex: NeuAcα2-3Galβ1-3(Fucα1-4)GlcNAcβ1-3(Galβ1-4(Fucα1-3)GlcNAcβ1-6)Galβ1-4Glcβ-Cer(d18:1/24 : 0) or sLea-x: NeuAcα2-3Galβ1-3(Fucα1-4)GlcNAcβ1-3Galβ1-4(Fucα1-3)GlcNAcβ1-3Galβ1-4Glcβ-Cer(d18:1/24 : 0) |
FA: fatty acid; Cer: ceramide; Gal:Galactose; Glc:Glucose, Lac: Lactose; Ac: acetyl; SM: sphingomyelins; NeuAc: N-acetylneuraminic acid; KDN: 2-keto-3-deoxy-D-glycero-D-galacto-nononic acid; NeuGc; N-Glycolylneuraminic acid.