Table 1.
Dynamic light scattering (DLS) assessment of lipid nanoparticles. (n = 3).
| PBS | ||||||||||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| 0% sucrose | 5% sucrose | 10% sucrose | 30% sucrose | |||||||||||||||||
| Temp | Fresh | 4 °C | −20 °C | −80 °C | −200 °C | Fresh | 4 °C | −20 °C | −80 °C | −200 °C | Fresh | 4 °C | −20 °C | −80 °C | −200 °C | Fresh | 4 °C | −20 °C | −80 °C | −200 °C |
| PDI ± st. dev |
0.233 ± 0.030 |
0.401 ± 0.016 |
0.273 ± 0.012 |
0.912 ± 0.153 |
0.282 ± 0.041 |
0.294 ± 0.060 |
0.357 ± 0.050 |
0.293 ± 0.072 |
0.818 ± 0.261 |
0.212 ± 0.018 |
0.292 ± 0.061 |
0.280 ± 0.027 |
0.273 ± 0.056 |
0.980 ± 0.035 |
0.171 ± 0.062 |
0.283 ± 0.049 |
0.336 ± 0.027 |
0.286 ± 0.063 |
0.688 ± 0.209 |
0.280 ± 0.092 |
|
Z-avg diam. ± st. dev (nm) |
114 ± 3 |
107 ± 40 |
125 ± 1 |
4037 ± 2267 |
141 ± 5 |
92 ± 18 |
95 ± 7 |
96 ± 6 |
1448 ± 781 |
124 ± 51 |
90 ± 7 |
88 ± 10 |
88 ± 13 |
3927 ± 4238 |
93 ± 21 |
96 ± 8 |
97 ± 24 |
86 ± 14 |
741 ± 498 |
83 ± 7 |
| TBS | ||||||||||||||||||||
| PDI ± st. dev |
0.293 ± 0.016 |
0.225 ± 0.008 |
0.246 ± 0.01 |
0.784 ± 0.201 |
0.255 ± 0.016 |
0.251 ± 0.021 |
0.257 ± 0.060 |
0.239 ± 0.071 |
0.787 ± 0.188 |
0.228 ± 0.047 |
0.231 ± 0.012 |
0.247 ± 0.036 |
0.225 ± 0.054 |
0.866 ± 0.155 |
0.235 ± 0.043 |
0.230 ± 0.036 |
0.281 ± 0.103 |
0.239 ± 0.088 |
0.907 ± 0.161 |
0.255 ± 0.042 |
| Z-avg diam. ± st. dev (nm) | 113 ± 11 |
159 ± 7 |
162 ± 5 |
1959 ± 786 |
155 ± 8 |
123 ± 17 |
113 ± 7 |
120 ± 17 |
1140 ± 590 |
120 ± 14 |
118 ± 12 |
114 ± 10 |
120 ± 17 |
1558 ± 1302 |
121 ± 24 |
119 ± 13 |
120 ± 15 |
122 ± 19 |
4856 ± 3625 |
118 ± 13 |