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. 2022 Nov 7;25(12):1597–1607. doi: 10.1038/s41593-022-01191-6

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© The Author(s) 2022

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Extended Data Fig. 3 — a, b, Immunofluorescence, merge and colocalization images of BSN (red) and pTauS396/S404 (PHF1, green) in human AD (from middle frontal gyrus, MFG) (a), and PSP (from pons) (b), and healthy control subjects from MFG and pons, respectively. Merge image includes orthogonal image of reconstructed three-dimensional views. Scale bar: 200 µm for main images and 50 µm for insets. Experiments were performed in triplicates for AD and PSP cases.