TABLE 1.
Bacterial strains and plasmids used in this study
| Strain or plasmid | Characteristicsa | Source or reference |
|---|---|---|
| E. coli DH5αF′b | F′ endA1 hsdR17 supE44 thi-1 recA1 gyrA relA1 Φ80lacZΔM15 Δ(lacZYA-argF) U169 | Life Technologies |
| B. suisc | ||
| 1330 | Biotype 1; ATCC 23444 | ATCCd |
| 1330-BBR1-KGFP | 1330 strain carrying pBBR1-KGFP; no gfp expression | This study |
| Plasmids | ||
| pUC4K | Contains Kanr cassette flanked by polylinker | Pharmacia |
| pBBR1MCS | Broad-host-range cloning vector (Cmr) | 8 |
| pFPV25 | Promoter trap vector containing gfpmut3 in plasmid pED350 (Ampr) | 18 |
| pBBR1-KGFP | pBBR1MCS derivative; Kanr; promoterless gfpmut3 from pFPV25 | This study |
a
Ampr, ampicillin resistance; Cmr, chloramphenicol resistance; Kanr, kanamycin resistance.
b
E. coli strains were grown in Luria-Bertani broth supplemented with the appropriate antibiotics at 37°C.
c
B. suis strains were grown at 37°C in TS supplemented with antibiotics when appropriate.
d
ATCC, American Type Culture Collection.