Table 5.
Multiplexes composition for PIK3CA mutations analysis.
| Multiplex | Forward primer | Probe | Reverse primer |
|---|---|---|---|
| ALB/R88Q/N345K/C420R | ALB-F | ALB-P | ALB-R |
| R88Q-F | R88Q-P | R88Q-R | |
| N345K–F | N345K–P | N345-R | |
| C420–F | C420–P | C420-R | |
| ALB/E542K/H1047L | ALB-F | ALB-P | ALB-R |
| E542K–F | E542K-R | E542K-2 | |
| Ex20-F1 | Ex20-P1 | H1047L-R | |
| ALB/E545K/H1047R | ALB-F | ALB-P | ALB-R |
| E542K–F | E542K-R | E545K-2 | |
| Ex20-F1 | Ex20-P1 | H1047R-R | |
| ALB//M1043I | ALB-F | ALB-P | ALB-R |
| Ex20-F2 | Ex20-P2 | M1043I-R | |
| ALB/Q546K/H1049Y | ALB-F | ALB-P | ALB-R |
| E542K–F | E542K-R | Q546K-R | |
| Ex20-F2 | Ex20-P2 | H1047Y-R |