Figure 3.

Per2 is required for LDR-induced mitochondrial activation (see also Figure S4)

(A) A cluster of genes related to mitochondrial metabolic functions by RNAseq analysis of Per2^wt versus Per2^def BM-HSCs with 1.2-fold cutoff.

(B) Gene ontology biological process enrichment analysis of up-regulated genes related to mitochondrial metabolic functions with 1.2-fold cutoff in Per2^wt versus Per2^def BM-pHSCs.

(C–F) Western blot of a cluster of mitochondrial metabolic factors CPT1A, CPT2, NDUFA12, and NDUFV3 in Per2^wt and Per2^def BMMNCs. Mitochondrial membrane potential (D), oxygen consumption (E), and ATP generation (F) were measured in Per2^wt and Per2^def BMMNCs 24 h after LDR.

Data are represented as mean ± SEM, n = 3, ∗p < 0.05; ∗∗p < 0.01, ns p > 0.05, Student’s t test.