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. 2022 Nov 12;25(12):105569. doi: 10.1016/j.isci.2022.105569

Figure 1.

Figure 1

Hepatocyte Gdf15 expression is sufficient for high-fat diet-induced increases in plasma GDF15

(A) Schematic of Gdf15 knockout-first strain and hepatocyte-specific Gdf15 re-expression model.

(B) Study group and timeline overview.

(C–H) Gdf15 mRNA levels relative to Actb expressed as fold-change relative to WT LFD for liver (C), white adipose tissue (D), brown adipose tissue (E), skeletal muscle (F), heart (G) and kidney (H).

(I) RNA in situ hybridization for Gdf15 exon two of liver samples from HFD-fed KO + GFP and KO + FLP mice.

(J) Photomicrographs of frozen liver sections from RosamTmG/mTmG mice after AAV8-TBG-CRE dosing.

(K) Plasma GDF15 levels. Data are the mean ± SEM for n = 6–9/group for (C, I and K), n = 4/group for (D–H). Scale bar in (I) and (J) represents 100 microns. Data analyzed by 1-way ANOVA with multiple comparison testing. ∗p < 0.05, ∗∗p < 0.01, ∗∗∗p < 0.001, ∗∗∗∗p < 0.0001.