REAGENT or RESOURCE	SOURCE	IDENTIFIER
Bacterial and virus strains
AAV8-TBG-GFP	Vector Biolabs	VB1728
AAV8-TBG-FLP	Vector Biolabs	VB1724
Chemicals, peptides, and recombinant proteins
3-3H-glucose	Perkin Elmer	NET331C001MC
Insulin	Novolin-R, Novo Nordisk	NDC 0169-1833-11
Dextrose	Sigma Aldrich	D9434
Critical commercial assays
Qiagen RNeasy kit	Qiagen	74104
Quantitect Reverse Transcriptase Kit	Qiagen	205313
PowerUp SYBR Green Master Mix	Thermo Fisher	A25778
GDF15 ELISA	R&D systems	MGD150
RNAscope 2.5 High Definition-BROWN assay	Bio-Techne	322300
Hematoxylin QS	Vector laboratories	H-3404-100
Total Cholesterol Kit, Wako Diagnostics	Fisher Scientific	NC9138103
NEFA HR(2) solvent B, Wako Diagnostics	Fisher Scientific	NC9517311
NEFA HR(2) solvent A, Wako Diagnostics	Fisher Scientific	NC9517309
NEFA HR(2) Color reagent B, Wako Diagnostics	Fisher Scientific	NC9517310
NEFA HR(2) Color reagent A, Wako Diagnostics	Fisher Scientific	NC9517308
Triglyceride Infinity Reagent	Thermo Fisher	TR22421
STELLUX Chemiluminescent Rodent Insulin ELISA	ALPCO Diagnostics	80-INSMR-CH10
Experimental models: Organisms/strains
Mouse: C57BL6/J mice (wildtype)	Jackson Laboratory	000664
Mouse sperm: Gdf15 knockout-first C57BL/6N-Gdf15tm1a(KOMP)Wtsi/H	Knock Out Mouse Project (KOMP)	EMMA Strain ID 06354
Mouse: RosamTmG; B6.129(Cg)- Gt(ROSA)26Sortm4 (ACTB-tdTomato,−EGFP)Luo/J	Jackson Laboratory	007676
Mouse: C57BL6/J diet-induced obese mice	Jackson Laboratory	380050
Oligonucleotides
Gdf15 primers Predesigned and validated by IDT	Integrated DNA Technologies (IDT)	Mm.PT.58.13112185
Actb; FWD GCAGCTCCTTCGTTGCCGGT; REV TACAGCCCGGGGAGCATCGT); Designed using IDT online software	IDT	N/A
Custom RNAscope probe targeting Gdf15 exon 2	Bio-Techne	300040
Software and algorithms
Prism	GraphPad Software	Version 9.3.1
Other
Mouse Diet: Regular chow diet	ProLab IsoPro	RMH 3000
Mouse Diet: control low-fat diet (LFD)	Research Diets	D12450J
Mouse Diet: experimental high-fat diet (HFD)	Research Diets	D12492