. Author manuscript; available in PMC: 2022 Dec 1.

Published in final edited form as: J Bone Miner Res. 2022 Jul 3;37(11):2077–2093. doi: 10.1002/jbmr.4617

Table 2.

Summary of Phenotypes of Different Genotypic Classes, as Assessed by ABAR Staining of P0 Mice

Phenotype	Wild type	R26 ^ACVR1/+	Acvr1 ^R206H/+	Acvr1^R206H/+; R26^ACVR1/+	Acvr1 ^R206H/−	Acvr1^R206H/−; R26^ACVR1/+
Found dead	1/18 (5.6%)	1/14 (7.1%)	3/20 (6.0%)	0/27 (0%)	10/10 (100%)	1/15 (6.7%)
Spina bifida	0/16 (0%)	0/13 (0%)	20/20 (100%)	0/27 (0%)	8/8 (100%)	0/15 (0%)
Costovertebral fusion	0/16 (0%)	0/14 (0%)	20/20 (100%)	0/27 (0%)	8/8 (100%)	0/15 (0%)
Intervertebral fusion	0/16 (0%)	0/14 (0%)	0/20 (0%)	0/27 (0%)	8/8 (100%)	0/15 (0%)
L₁ supernumerary rib	2/16 (12.5%)	5/14 (35.7%)	16/20 (80%)	13/27 (48.1%)	8/8 (100%)	9/15 (60.0%)
Zeugopod truncation	0/18 (0%)	0/14 (0%)	20/20 (100%)	0/27 (0%)	9/9 (100%)	0/15 (0%)
Metacarpal/metatarsal-phalangeal fusion	0/18 (0%)	0/13 (0%)	20/20 (100%)	0/27 (0%)	9/9 (100%)	0/14 (0%)

Differences in numbers of mice scored within a group reflect damage during processing and inability to score a particular phenotype. For Acvr1^R206H/− genotypes, the null allele was either the unrecombined Acvr1^tnR206H allele or the germline-recombined Acvr1^loxP allele.