TABLE 1.
Bacterial strains used in this study
| Strain | Descriptiona | Source or reference |
|---|---|---|
| MC1000 | E. coli K-12, F−galK16 galE15 relA1 rpsL150 spoT1 mcrB1 | 7 |
| C600 | E. coli K-12, F−thi-1 thr-1 leuB6 lacY1 tonA21 supE | 17 |
| C600(933W) | Lysogen of O157:H7-derived Stx2-encoding phage 933W | 31 |
| C600(λcI857) | Lysogen of phage λ with heat-labile repressor mutation cI857 | This study |
| C600(H19B) | Lysogen of Stx1-encoding lambdoid phage H19B | 18 |
| STEC clinical isolates | ||
| 933 | Oregon, 1982; serotype O157:H7 | 31 |
| STEC-10 | South Dakota, 1983; serotype O145:NM | 75-83(CDC)b |
| STEC-2 | Ohio, 1997; serotype O157:H7 | 9:100 (1) |
| STEC-3 | Michigan, 1997; serotype O157:H7 | 1:361 (1) |
| STEC-14 | Serotype O157:H7 | B94007314(CDC) |
| STEC-16 | Nebraska, 1985; serotype O111:NM | 3007-85(CDC) |
| STEC-19 | Ohio, 1990; serotype O83:H1 | 3119-90(CDC) |
| MC1000 lysogens | ||
| MC1000(933W) | Phage isolated from 933 (O157) | This study |
| MC1000(φ10) | Phage isolated from STEC-10 (O145) | This study |
| MC1000(φ2) | Phage isolated from STEC-2 (O157) | This study |
| MC1000(φ3) | Phage isolated from STEC-3 (O157) | This study |
| MC1000(φ14) | Phage isolated from STEC-14 (O157) | This study |
| MC1000(φ16) | Phage isolated from STEC-16 (O111) | This study |
| MC1000(φ19) | Phage isolated from STEC-19 (O83) | This study |
| MC1000(λcI857) | Lysogen of phage λ with heat-labile repressor mutation cI857 | This study |
| MC1000(H19B) | Lysogen of Stx1-encoding lambdoid phage H19B | This study |
a
For clinical isolates, date and location of isolation and serotype are given when known. For MC1000 lysogens, the strain from which each phage was isolated is given.
b
CDC indicates strains acquired from the Centers for Disease Control and Prevention.