Fig. 4. Effect of fulvestrant and elacestrant on the ER-binding in MCF7, MCF7-LTED^wt and MCF7-LTED^Y537C.

A ChIP-seq revealing similar effects of elacestrant (EL) and fulvestrant (FULV) on ER binding. Significantly differential ER binding (FDR < 0.05) as determined by DiffBind between EL + E2 and E2-only (red), between FULV + E2 and E2-only (blue) and overlap of significant binding sites (purple). B Genomic distribution of ER-binding sites. C RIME proteomics analysis revealing a similar effect of elacestrant (EL) and fulvestrant (FULV) on the ER-interactome. Different colour dots correspond to Log2 differences. D Heatmap showing GO hormone-binding proteins that interacted in cells treated with fulvestrant or elacestrant in relation to the control (E2). p value based on Student’s t-test or Pearson correlation is shown; r Pearson’s correlation score (Data from n = 2 biological replicates per treatment per cell line).