Table 2.

Orthogonal experimental design.

NO.	Factor
	A: Inoculation amount v/v%	B: pH	C: Temperature °C	TRP concentration ng/ml
1	2	5.8	30	15.5
2	2	6.8	37	9.54
3	2	7.8	40	2.71
4	4	5.8	37	12.7
5	4	6.8	40	6.36
6	4	7.8	30	12.1
7	6	5.8	40	9.67
8	6	6.8	30	14.3
9	6	7.8	37	11.3
K1	27.75	37.87	41.9
K2	31.16	30.2	33.54
K3	35.27	26.11	18.74
Ki	9.25	12.62	13.97
Kii	10.37	10.07	11.18
Kiii	11.76	8.70	6.25
R	2.51	3.92	7.72