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. 2022 Nov 29;13:7341. doi: 10.1038/s41467-022-35127-2

Fig. 2. iDuo-MM CAR-NK cells with the anti-BCMA CAR4 construct mediate sustained cytotoxic activity against MM.1S cells in a serial restimulation assay.

Fig. 2

A serial restimulation cytotoxicity assay was performed with MM.1S cells transduced with NucLight Red as targets in the presence or absence of daratumumab, using backbone iNK cells or iDuo-MM CAR-NK cells as effectors. MM.1S cells were plated on the day of assay initiation followed by the addition of iNK cells at a 1:1 E:T ratio. After 48 hours of co-culture (round 1), non-adherent effector cells were transferred to new plates containing freshly plated MM.1S target cells (round 2) without recalibration of the E:T ratio. This procedure was repeated for a third round (round 3) of stimulation. MM.1S target cell survival was assessed in real-time using IncuCyte imaging. Data are representative of two independent experiments. Source data are provided as a Source Data file.