Figure 6.

Ex vivo analysis of cell proliferation index and blood vessel density of therapy tumors

Frozen tissue sections from GBM of the therapy study were prepared and stained for Ki67 (green) for cell proliferation index and CD31 (red) for blood vessel density. Hoechst (blue) was used for nuclei staining. The two therapy groups Dual/NIS (A) and Ge11/NIS (B) followed by ¹³¹I showed significantly fewer Ki67-positive cells and significantly smaller CD31-positive area compared with TfRre/NIS + ¹³¹I (C) and negative control groups: Dual/LUC + ¹³¹I (D), Dual/NIS + NaCl (E), NaCl only (F). Quantitative analysis for cell proliferation index (G) and blood vessel density (H) are shown (∗p ≤ 0.05, ∗∗p ≤ 0.01, ∗∗∗p ≤ 0.001). One representative picture of each group is shown at 20× magnification (scale bar, 50 μm). Results are reported as mean ± SEM (for each group n = 4).