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. 2022 Nov 16;9:1051157. doi: 10.3389/fnut.2022.1051157

FIGURE 1.

FIGURE 1

NASH is associated with skeletal muscle alterations. (A) Representative hematoxylin-eosin (H&E) and Sirius red (SR) staining of liver sections from wild-type normal diet (WT ND), WT high-fat fed (WT HFD), and HFD-fed fat Aussie (FOZ HFD) mice after 12 and 20 weeks of diet; (B) Non-alcoholic fatty liver disease activity score (NAS) (n = 4–9); (C) Collagen area assessed by digital analysis in the liver of WT ND, WT HFD and FOZ HFD after 12 and 20 weeks of HFD (n = 7–9); (D) Liver-to-spleen density measured in vivo by micro-CT in WT ND, WT HFD, and FOZ HFD after 0, 8, 12, and 20 weeks of diet (n = 4–10); (E) Fat mass and (F) fat-free mass (i.e., lean body mass including muscles and organs, bones excluded) measured in vivo by micro-CT (n = 6–9); (G) Dorsal muscle area (L4–L5 averaged) in WT ND, WT HFD, and FOZ HFD between 0 and 20 weeks of diet (n = 6–10). The * represents the significant difference between 12 W and 20 W in FOZ HFD; (H) Dorsal muscle-to-spleen density measured in vivo by micro-CT in WT ND and FOZ HFD (n = 6–10); (I) 4-limbs grip strength measured in WT ND, WT HFD, and FOZ HFD between 0 and 20 weeks of diet (n = 8–18). The ** represents the significant difference between FOZ HFD and the two other groups at 20 W. All data are represented as mean ± SD, *p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001. Statistical tests used: (B,C,E,F) one-way ANOVA and (D,G,H,I) two-way ANOVA followed by post hoc Bonferroni correction.