Figure 1.

Generation of Gata3-ZsGreen reporter system on WT (Gata3 ^ZsG) and on conditional Gata3 KO (Gata3 ^ZsG-fl) background. (A) Schematic representation of steps involved in the generation of mouse strains carrying the Gata3 ^ZsG or Gata3 ^ZsG-fl allele from the initial Gata3 ^ZsG-BAC construction to CRISPR/Cas9 targeted integration of ZsGreen-T2A reporter into the mouse genome. (B) Expected expression of ZsGreen reporter and functional GATA3 protein from the same mRNA. (C) Expected expression of ZsGreen reporter and non-functional GATA3 protein after exon 4 deletion. (D) Gel image of genotyping results distinguishing the Gata3 ^ZsG reporter allele from the WT Gata3 ⁺ allele. (E) Flow cytometry analysis of ZsGreen expression among CD4⁺ T cells, CD8⁺ T cells, and B lymphocytes in the blood. The results (D, E) are representative of more than two independent experiments.