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. 2022 Nov 16;13:975958. doi: 10.3389/fimmu.2022.975958

Figure 5.

Figure 5

ZsGreen continues to be expressed in the GATA3 KO “Th2” cultures. (A) Schematic diagram for Th2 cell differentiation using naïve CD4 T cells from Gata3 fl/flCreERT2, Gata3 fl/fl and Gata3 ZsG-fl/flCreERT2 mice followed by 4-HT treatment to delete Gata3 gene in CreERT2 positive cells. (B) Flow cytometry histograms showing GATA3 protein levels and ZsGreen reporter expression from Th2 cells with and without 4-HT treatment. (C) qPCR data for analyzing the expression of ZsGreen mRNA and mRNA of indicated Gata3 exons in the presence and absence of 4-HT (Mean ± SEM; n = 6; ****p < 0.0001, Student t-test). (D) Flow cytometry analysis of IL-4 and IL-13 expression by in vitro differentiated Th2 cells followed by 4-HT treatment. (E) The IL-4+ and IL-13+ Th2 cell population from (D) were counted and plotted (Mean ± SEM; n = 3-8; ns, not significant; ****p < 0.0001, Student t-test). Results are representative of two independent experiments.