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. 2022 Nov 16;13:975958. doi: 10.3389/fimmu.2022.975958

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Copyright © 2022 Gurram, Wei, Yu, Kamenyeva, Chung, Zheng, Butcher, Kabat, Liu, Khillan and Zhu

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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GATA3-deficient “ILC2s” are relatively stable in vivo. (A) Mice were challenged (i.n.) with papain and injected (i.p.) with tamoxifen as indicated in the figure for different durations. The mice were euthanized on day 16 to analyze the ILC2s and Th2 cells in the lung. (B) Gating strategy for identifying ILC2s and Th2 cells. (C) Flow cytometry data showing the ILC2s and Th2 cells at various time duration after TMX injection. (D) The total numbers of ILC2s and Th2 cells in (C) were counted, and the values were plotted. Results are representative of two independent experiments.