Figure 1.

Loss of topologically associating domains during mouse terminal erythropoiesis

(A) Schematic overview of the mouse primary fetal liver culture system and overall experimental design.

(B) Hi-C contact matrices of chromosome 2 (35-85Mb) at 100 kb resolution in freshly isolated cells (0 h) and cells cultured in EPO containing medium for 30 h.

(C) Measurement of Hi-C genomic contact distance in cells from B.

(D) Number of TADs identified in the indicated cells.

(E) The distribution of TAD length in the indicated cells.

(F) The insulation scores of TADs identified in the indicated cells.

(G) Aggregated Hi-C interaction frequencies and the neighboring regions (+/− 0.5 TAD length) in the indicated cells.

(H) Representative Hi-C maps on regions chr3:41-51Mb and chr4:74-83Mb with disrupted chromatin domains in cells at 30 h group.